SUMO化蛋白质组的富集策略研究进展

李洋; 单亦初; 梁振; 张丽华; 张玉奎

Research Advances of Enrichment Approaches for SUMOylated Proteome

DOI：

KeyWord:SUMOylation deep coverage enrichment detection

Author	Institution
LI Yang,SHAN Yi-chu,LIANG Zhen,ZHANG Li-hua,ZHANG Yu-kui	1. Key Laboratory of Separation Science for Analytical Chemistry，Dalian Institute of Chemical Physics，Chinese Academy of Sciences，Dalian ，China; 2. University of Chinese Academy of Sciences，Beijing ，China; 3. Institute of Drug Discovery Technology，Ningbo University，Ningbo ，China

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Abstract:

Protein SUMOylation is one of the most important post-translational modifications，which involved in various biological processes，including nuclear transport，transcriptional regulation，maintenance of gene integrity and cell cycle．However，identification of SUMOylation has always been the hotspot and difficulty in this field due to the low abundance and lengthy peptide sequence of endogenous SUMOylation．Therefore，the efficient enrichment of SUMOylated proteins/peptides is necessary to achieve deep coverage analysis of SUMOylome．The basic principles，characteristics and latest progresses of different enrichment methods for SUMOylated proteome are reviewed，and the development prospects are prospected.