CRISPR/Cas Based Biosensing Platforms for Molecular Diagnosis
  
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KeyWord:CRISPR/Cas  ?molecular diagnosis  ?point-of-care testing  ?biosensing
  
AuthorInstitution
LI Tian,LI Na 1. School of Basic Medical Sciences,Henan University of Science and Technology,Luoyang ,China;2. College of Chemistry and Molecular Engineering,Peking University,Beijing ,China
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Abstract:
      Clustered regularly interspaced short palindromic repeats(CRISPR) and CRISPR-associated proteins(Cas) have become highly promising candidates in the construction of biosensing systems and diagnostic devices,in addition to their roles as revolutionary genome engineering tools.For?in vitro diagnostics,the class Ⅱ CRISPR/Cas systems possess unique advantages,such as high detection sensitivity and specificity,and the feasibility for developing point-of-care diagnostic technology.This minireview briefly introduces the working mechanism of CRISPR/Cas systems and mainly focus on the various diagnostic methods based on the CRISPR/Cas for detecting different forms of the genetic molecules in recent three years according to different readout methods.
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