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| Study on Efficient Hydrolysis of Pyrethroid Pesticide Using a Cross-linked “Nanoflower” Enzyme |
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| DOI： |
| KeyWord:glutaraldehyde cross-linking pyrethroid pesticide digestion storage stability reusability |
| Author | Institution |
| LIU Yu，YE Tai，CAO Hui，YUAN Min，YU Jin-song，XU Fei，ZHU Jia-yi，QI Hong，CHEN Jia-yu |
Shanghai Engineering Technology Research Center for Rapid Food Detection，School of Health Science and Engineering，University of Shanghai for Science and Technology，Shanghai ，China |
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| Abstract: |
| Cross-linked porcine liver esterase hybrid“nanoflower”enzyme（CL－PLE－NF） was prepared via biomineralization coupling wet cross-linking，and the preparation conditions were optimized. Under the optimum conditions，the activity and maximum reaction rate of CL－PLE－NFs exhibited 231% and 134% those of free enzyme，respectively．After stored at 4 ℃ for 320 days，the CL－PLE－NFs still retained 70.85% of initial activity，which indicates its good storage stability．Additionally，pyrethroid pesticides were employed to investigate the hydrolysis performance of CL－PLE－NFs．It was found that eleven kinds of pyrethroid pesticides，including type Ⅰ and type Ⅱ，gave over 55% hydrolysis efficiency in 5 min．After 12 cycles of use，the hydrolysis efficiency of Cl－PLE－NFs for detamethrin still maintained 65.37%． |
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