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| Research on Detection Methods and Biology of DNA Abasic Sites |
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| DOI： |
| KeyWord:abasic sites DNA damage mass spectrometry DNA interstrand crosslink DNA－protein crosslink |
| Author | Institution |
| WU Hai-jiang，ZHANG Ya-jiao，YUAN Shu-yan，XU Hua，XIE Jian-wei |
1. State Key Laboratory of Toxicology and Medical Countermeasures，Institute of Pharmacology and Toxicology，Academy of Military Medical Sciences，Beijing ，China; 2. Wuya College of Innovation， Shenyang Pharmaceutical University，Shenyang ，China |
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| Abstract: |
| The abasic sites（apurinic/apyrimidinic sites，AP sites） generated from the cleavage of N-glycosylic bond in DNA are one of the common lesions in DNA，which are formed via spontaneous hydrolysis，as well as a major intermediate during the base excision repair．If AP sites are not repaired timely，they may cause DNA replication stalling and DNA strand breaks，resulting in mutagenesis and cytotoxicity，and further cause DNA crosslinks or DNA-protein crosslinks．Therefore，analysis of AP sites will be helpful for the assessment of cellular oxidative stress damage or the toxicity evaluation of genotoxic chemicals．A variety of analytical methods have been developed for the detection of AP sites in DNA，including 14C or 32P postlabeling，enzyme-linked immunosorbent assay（ELISA） and liquid chromatography－mass spectrometry（LC－MS）．The research progress in the detection methods and biology of AP sites in DNA is reviewed in this paper，and the research prospects of AP sites are presented． |
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