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| Study on Separation and Purification of Chlorogenic Acid from Honeysuckle Flower by Macroporous Resin |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:honeysuckle flower chlorogenic acid macroporous resin static adsorption dynamic adsorption separation and purification |
| Author | Institution |
| GONG Zhihua1,2,REN Guopu3,SHU Qingxiao4,XIAO Wenjun1,2,4 |
1.湖南农业大学园艺园林学院茶学系;2.湖南农业大学国家植物功能成分利用工程技术研究中心;3.中南林业科技大学食品科学与工程学院;4.湖南农业大学园艺园林学院植物资源工程系 |
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| Abstract: |
| The tests of single factor,orthogonal and verification to separate and purify chlorogenic acid from honeysuckle crude extracts by NKA 2，D1400，polyamide，HP2MGL，ADS 21，D101 and AB 8 seven macroporous resin were carried out in sequence,and the best processing parameters were optimized based on the adsorption and desorption results.The results showed that NKA 2 was the best resin,whose static saturated adsorption capacity was 21217 mg/g,and desorption rate by 90% ethanol reached 814%.The best processing parameters for separating and purifying chlorogenic acid from honeysuckle crude extracts by NKA 2 resin were as follows:sample concentration:4 g/L,sample volume:35 BV,sample flow rate:3 BV/h,elution ethanol concentration:80%,elution volume:3 BV,and elution flow rate:1 BV/h.Under the optimal conditions,the yield rate of chlorogenic acid reached 8579%,and its purity was 4550%.When using sample concentration of 3 g/L,sample volume of 45 BV,sample flow rate of 2 BV/h,elution ethanol concentration of 80%,elution volume of 3 BV and elution flow rate of 3 BV/h,the purity of chlorogenic acid reached 5307%,and its yield was 7333%.Comparing with the current separating and purifying process of chlorogenic acid from honeysuckle crude extracts,the optimized process has better practical and reference value. |
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