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| Determination of Polyoxin B in Soils by Capillary Electrophoresis with Electrochemiluminescence Detection |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:capillary electrophoresis electrochemiluminescence polyoxin B soil |
| Author | Institution |
| 李玉杰，周敏，马永钧，司长代，米娟，刘彩云，陈慧 |
西北师范大学化学化工学院甘肃省高分子材料重点实验室省部共建生态环境相关高分子材料教育部重点实验室 |
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| Abstract: |
| On the basis of a chemically modified platinum electrode by europium （Ⅲ）-doped Prussian blue analogue film（Eu-PB） as a working electrode，a rapid and sensitive method for the determination of polyoxin B was established using capillary electrophoresis coupled with electrochemiluminescence detection（CE-ECL）.Parameters related to the separation and detection were discussed and optimized.The possible mechanism of ECL was briefly discussed.Under the optimized conditions（ECL detection potential at 1.27 V，electrokinetic injection for 10 s at 8.5 kV，16 kV as the separation voltage，20 mmol/L pH 8.5 phosphate buffer containing 16 % acetonitrile as running buffer），polyoxin B was separated within 4 min，and the ECL intensity was in proportion to logarithm of polyoxin B concentration over the range of 1.0×10-4-5.0×10-4 g/L and 5.0×10-4-5.0×10-2 g/L with a detection limit(3σ) of 9.2×10-5 g/L.The relative standard deviations（RSDs） of intra day and inter day ECL intensity and migration time were all less than 5.0%.The proposed method was successfully applied in the determination of polyoxin B absorbed in three kinds of soils（egbentonite，kaolinite and loess） with the recoveries of 92%-104%.The results also showed that the adsorption rate of polyoxin B in soils for 2 h was 89.0% for bentonite，45.5% for kaolinite and 40.4% for loess，respectively，indicating that the adsorption capacity of polyoxin B in different soils was bentonite>kaolinite>loess.The possible reason was related to the characteristics，specific surface area and cation exchange capacity（CEC） of soils. |
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