| With the accomplishment of the gene sequencing of Human Genomic Project(HGP）,increasing attention is focusing on proteomic research.Glycosylation of proteins is the most common and important post translational modification,which is composed of many covalent forms of polypeptide and oligosaccharide.The structure of sugar chain plays a crucial role in various biological processes.However,due to the existence of high abundant non glycoproteins,the low abundant glycoproteins were prone to be embedded and covered by nonglycoproteins and thus causing serious interference in further identification.The existing technologies are difficult to isolate and detect low abundant glycoproteins from complex matrices,which has become the one of the major bottlenecks currently encountered in glycoproteomics.Monolithic column,as an integrated continuous porous stationary phase,was prepared by in situ polymerization or sol-gel process inside the capillary,which can be taken as an alternative approach for isolation and enrichment of glycoproteins due to the merits of simple preparation procedure,high porosity,high capacity and high efficient separation.In this paper,the principle,preparation and classification of monolithic columns were briefly introduced.The recent advances of monolithic columns in selective separation and enrichment of glycoproteins were summarized,which included lectin based affinity monoliths,boronate affinity monoliths and immobilized metal affinity monoliths.In addition,the prospect of monolithic column for enrichment of glycoproteins was also presented.Totally,65 references were reviewed. |