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| Determination of Psoralen and Bergapten in Radix fici hirtae by Liquid-Liquid Microextraction Coupled with Liquid Chromatography-Mass Spectrometry |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:Radix fici hirtae liquid-liquid microextraction（LLME） high performance liquid chromatography-mass spectrometry（HPLC-MS） psoralen bergapten |
| Author | Institution |
| 陶敬奇,闻莹莹, 王辉,王三永,陈洪伟 |
1.华南师范大学化学与环境学院， 2.广东省食品工业研究所， 3.东莞理工学院化学与环境工程学院 |
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| Abstract: |
| A high performance liquid chromatography coupled with electro spray ionization tandem mass spectrometric（HPLC-ESI MSn） method was developed for the simultaneous determination of psoralen and bergapten in aqueous extraction from Radix fici hirtae.The separation of psoralen and bergapten was performed on a Hypersil C18 column（150 mm×4.6 mm,5 μm）,using water-methanol（50∶50）as mobile phase at a flow rate of 0.80 mL·min-1.The detection was carried out at room temperature with a detection wavelength of 300 nm（PDA） and an injection volume of 10 μL.The influencing factors for liquid-liquid microextraction（LLME） were investigated.Under the optimized conditions,the calibration curves were linear in the range of 0.55-2 200 μg·L-1 for psoralen and 1.00-4 000 μg·L-1 for bergapten,with correlation coefficients of 0.999 9 and 0.999 2,respectively.The limits of detection（S/N=3） were 0.13 μg·L-1 and 0.18 μg·L-1，and the limits of quantitation（S/N=10） were 0.43 μg·L-1 and 0.60 μg·L-1, respectively.The relative standard deviations（RSDs,n=5） of 110 μg·L-1 psoralen and 100 μg·L-1 bergapten were both less than 1%.The recoveries of psoralen and bergapten were 100% and 101%,respectively.The results demonstrated that LLME combined with LC-MS was a rapid,convenient and efficient method for the determination of herbal compositions,and could provide the basis for the quantitation detection of psoralen and bergapten in aqueous sample from Radix fici hirtae. |
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