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| Investigation on the Isolation and Purification of EGCG3″Me by High Speed Countercurrent Chromatography |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:EGCG3″Me high speed countercurrent chromatography isolation and purification high performance liquid chromatography |
| Author | Institution |
| 罗正飞，金孝芳，龚正礼，梁名志，王立波，陈继伟 |
1.西南大学食品科学学院，2.云南省农业科学院茶叶研究所，3.湖北省农业科学院果树茶叶研究所 |
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| Abstract: |
| A method for the isolation and purification of （-)-epigallocatechin-3-（3″-O-methyl)gallate（EGCG3″Me) from crude extract sample was firstly developed by high speed countercurrent chromatography(HSCCC).The results showed that HSCCC had a two phase solvent system composing of water-methanol-ethyl acetate-hexane（5∶2∶9∶1,by volume) on a preparative scale.The upper phase was used as the stationary phase,and the lower phase was used as the mobile phase.The revolution speed was 700 r/min,the flow rate was 2.0 mL/min and the detection wavelength was set at 254 nm.Under the optimized conditions,EGCG3″Me can be well isolated.The purity of EGCG3″Me detected by high performance liquid chromatography was more than 90%,and the yield of EGCG3″Me by HSCCC was 76.53%. |
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