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| Determination of Residues of Carbadox，Quinocetone，Mequindox and Their Metabolites in Aquatic Products by Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:UPLC-MS/MS aquatic products carbadox quinocetone mequindox metabolites |
| Author | Institution |
| 徐英江，任传博，田秀慧，邹荣婕，宫向红，张秀珍，张华威，于召强，刘永明 |
1.山东省海洋水产研究所;2.山东省海洋生态修复重点实验室;3.烟台大学化学生物理工学院;4.烟台山水海产有限公司 |
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| Abstract: |
| An ultra performance liquid chromatography-electrospray ionization triple-quadruple tandem mass spectrometric（UPLC-ESI MS/MS) method was established for the determination of residues of carbadox（CBX)，quinocetone（QCT)，mequindox（MEQ)，quinoxaline-2-carboxylic acid（QCA) and 3-methylquinoxaline-2-carboxylic acid（MQCA) in aquatic products.The samples were separately extracted with acetonitrile-ethyl acetate（50∶50) mixed solution and phosphate buffer，then purified with n-hexane.The separation of CBX，QCT，MEQ，QCA and MQCA was carried out on an ACQUITYTM BEH C18 column（1.7 μm，2.1 mm i.d.×100 mm) using methanol-acetronitril（11∶3) and 0.1% formic acid as mobile phases.The target compounds were confirmed and quantified by MS-MS under multiple-reaction monitering（MRM) mode with the external standard method.The results showed that there were good linear relationships between peak area and concentrations of five quinoxalines in the range of 5-50 μg/L，with correlation coefficients more than 0.998 and limits of quantitation（LOQs) of 1.0 μg/kg.The recoveries at spiked levels of 1.0-10 μg/kg ranged from 70% to 108% with relative standard deviations of 2.5%-11%.This method shortened the time of pre-treatment process，and the result was credible.It could satisfy the requirement of mass and fast analysis in the laboratory. |
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