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| Identification of Irradiated Protein rich Food by Dissociative Tyrosine |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:dissociative tyrosine irradiated food HPLC identification |
| Author | Institution |
| 吴敏，李久兴，张志刚，周昱，徐敦明 |
1.厦门出入境检验检疫局;2.厦门大学化学生物系 |
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| Abstract: |
| A novel method was presented for identification of irradiated protein-rich food by HPLC with dissociative tyrosine.The procedure involved the homogenization of fresh sample with 0.1 mol/L HCl，followed by centrifugation to remove the cell debris and the solvent precipitation of protein with acetone at -20 ℃，then the tyrosine was separated by forming ion pair with trifluoroacetic acid on a C18 column.The average recoveries of the drugs ranged from 61% to 116% at the spiked levels of 100-800 μg/L.The method is simple and double-quick,and could be efficiently identified food sample irradiated at a dose of 5 kGy or above. |
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