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| Determination of ｏ-Tyrosine in γ-Ray Irradiated Protein-rich Food by HPLC-MS/MS |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:ｏ-tyrosine γ-ray irradiated protein-rich food HPLC-MS/MS |
| Author | Institution |
| 王敏，张秀芹，杨振宇，仲维科，陆地，郑文杰 |
1.上海出入境检验检疫局;2.中国检验检疫科学研究院;3.烟台出入境检验检疫局;4.天津出入境检验检疫局 |
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| Abstract: |
| A liquid chromatography-tandem mass spectrometric method was developed for the determination of ｏ-tyrosine in γ-ray irradiated protein-rich food.The sample was homogenized and hydrolyzed with parenzyme at 37 ℃ for 24 h.The digestion solution was purified with n-hexane eliminating fat，deproteinated with zinc acetic acid solution，and then determined by HPLC-MS/MS.The quantitation limit was 0.1 mg/kg.The recoveries of ｏ-tyrosine at three spiked levels of 0.1，0.5 and 1.0 mg/kg were in the range of 80%-97%，with RSDs（n=6） of 6.3%-12.9%.The ｏ-tyrosine contents in chicken，pork，beef，fish and shrimp irradiated with 2，5,10 kGy of γ-ray ranged from 0.095 mg/kg to 0.677 mg/kg，which were obviously higher than those in the unirradiated meat samples,and presented linear relationships with the irradiance dose.The method showed good reproducibility and sensitivity. |
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