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| Determination of Four Polyphenolic Compounds in Pomegranate Peel Water Extracts by High Performance Liquid Chromatography |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:punicalagins A & B gallic acid ellagic acid pomegranate peel water extracts high performance liquid chromatography |
| Author | Institution |
| 曲文娟，张渭洁，徐敏瑶，杨洋，蒋洁玮 |
江苏大学食品与生物工程学院 |
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| Abstract: |
| To quantitative analyzing four polyphenolic compounds of gallic acid,punicalagin A,punicalalgin B and ellagic acid in pomegranate peel water extracts，a high performance liquid chromatographic（HPLC） method was established.The samples were prepared and filtered prior to the HPLC analysis.Quantification analysis of these analytes were achieved under the optimized HPLC conditions using the external standard method.The results showed that the calibration curves for gallic acid,punicalagin A，punicalagin B and ellagic acid were linear in the concentration ranges of 7.48-149.50，15.36-153.55，27.65-276.45，7.13-114.00 mg/L，respectively，with their correlation coefficients（r2） greater than 0.99.The limits of detection（LOD） were 0.12，1.92，3.46,0.22 mg/L，and the limits of quantitation（LOQ） were 0.48，7.68，13.83,0.89 mg/L，respectively.The spiked recoveries ranged from 83% to 112% with relative standard deviations（RSDs） less than 10%.The developed method was simple，sensitive，reliable and reproducible，and could be applied in the rapid determination of gallic acid，punicalagin A，punicalagin B and ellagic acid in pomegranate peel water extracts in 17 min. |
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