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| Rapid Detection of Chromium Ion by Gold Immunochromatographic Assay |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:chromium ions colloidal gold immunochromatography assay water sample monoclonal antibody |
| Author | Institution |
| 刘茜，唐勇，向军俭，邹军辉，翟一凡 |
广东省分子免疫与抗体工程重点实验室，暨南大学抗体工程研究中心 |
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| Abstract: |
| An immunochromatographic assay（ICA） method for the detection of chromium ions（Cr3+） was developed.Colloidal gold particles coated with affinity purified monoclonal anti isothiocyanobenzyl-EDTA（iEDAT）-chelated Cr3+ antibodies were used as detection reagent，and the strip was prepared by arraying the synthetic Cr3+-EDTA-BSA and goat anti-mouse immunoglobulins on cellulose nitrate film to form the test（T） band and control（C） band.The prepared strip was used to detect Cr6+ when it was reduced to Cr3+ by NaHSO3.The visual lowest detection limities（LDL） of the test strip was as low as 50 μg/L.The test trips showed negligible cross-reactivities with 10 heavy metals except for 1 000 μg/L Fe3+-EDTA.The determination result of water samples containing chromium standard by the prepared strip was consistent with that by ICP.Because of its sensitivity，specifity，low cost and fast result（within 5 min），the test strip is especially suitable for the on-site large scale screening on Cr-contained water samples and many other applications. |
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