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| Determination of Ractopamine and Clenbuterol in Processed Meats by High Performance Liquid Chromatography-Tandem Mass Spectrometry |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:high performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS) processed meat processing method ractopamine clenbuterol |
| Author | Institution |
| 汪辉，彭新凯，王玉枝，夏立新，曾欢 |
1.长沙市食品质量安全监督检测中心;2.湖南大学化学化工学院，化学生物传感与计量学国家重点实验室 |
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| Abstract: |
| A high performance liquid chromatography-tandem mass spectrometric(HPLC-MS/MS) method was developed for the simultaneous determination of ractopamine and clenbuterol in processed meats.A new cation exchange chromatographic column was utilized to separate target compounds and an improved pre-treatment method was uesed in this study.The eluent solution types and chromatographic parameters were optimized. The optimal conditions were as follows: using a flow rate of 0.4 mL?min-1，a injection volume of 10 μL and a column temperature of 40 ℃.The homogenized samples were extracted with 0.02 mol?L-1 ammonium acetate(pH 5.2)，hydrolyzed with 30 μL β-glucuronidase/arylsulfatase，centrifuged，and cleaned through solid phase extraction column. The calibration curves showed good linearities for two target compounds in the range of 0.1-100 μg?L-1.The limits of detection and quantitation of two target compounds were not more than 0.04 μg/kg and 0.15 μg/kg，respectively.The spiked recoveries for the processed meats ranged from 72% to 98% with RSDs less than 8.5%.This proposed method was simple and sensitive,and was suitable for the quantification of ractopamine and clenbuterol in processed meats. |
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