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| Fingerprint Analysis of Wanglaoji Guangdong Herbal Tea Granule by Ultra High Performance Liquid Chromatography |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:ultra high performance liquid chromatography fingerprint similarity evaluation principal component analysis Guangdong herbal tea granule quality control |
| Author | Institution |
| 杨运云，邓洁薇，余彦海，钟新林 |
1.中国广州分析测试中心广东省分析测试技术公共实验室;2.赛默飞世尔科技应用研究中心广州实验室 |
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| Abstract: |
| A fingerprint analysis method was developed for the quality control of Wanglaoji Guangdong herbal tea granule(GHTG)by ultra high performance liquid chromatography(UHPLC).The samples were extracted for 30 min by ultrasonic extraction using methanol as solvent.UHPLC separation was performed on a Waters ACQUITY HSS T3 C18(3.0 mm× 150 mm，1.8 μm) column by gradient elution with 0.5% formic acid-acetonitrile as mobile phase，at the temperature of 35 ℃ and a flow rate of 0.8 mL/min.A good separation of 28 common peaks was achieved within 18 min，and 4 of them were confirmed by reference substances.Similarity evaluation was performed by a professional software named Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(Version 2004A)，and the similarity among 23 batches of samples was no less than 0.95.The principal component analysis(PCA)also showed that the 23 batches of samples were located in one area.The results of similarity evaluation and PCA confirmed that the quality of Wanglaoji GHTG was stable，and the developed fingerprint analysis was fast，efficient and robust for the quality control of Wanglaoji GHTG. |
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