|
| A Fast Method for the Simultaneous Determination of Salivary Nicotine and Its Metabolites at Different Smoking Levels by Liquid Chromatography-Tandem Mass Spectrometry |
| |
| View Full Text Download reader |
| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:LC-MS/MS saliva nicotine cotinine half-life biomarker |
| Author | Institution |
| 王晔,周宛虹,金永明,郑赛晶 |
上海烟草集团有限责任公司技术中心 |
|
| Hits: 2308 |
| Download times: 999 |
| Abstract: |
| A fast,simple method for the determination of nicotine and its metabolites in human saliva was developed and validated by liquid chromatography-tandem mass spectrometry（LC-MS/MS）.Human saliva was collected with Salivette- collection kit and the quantification of analytes was achieved by using nicotinemethyl-d3,cotinine-methyl-d3 and trans-3′-hydroxycotinine-methyl-d3 as internal standard under atmosphere pressure chemical ionization（APCI） mode.The pretreatment of this method was simple and fast,and all analytes were eluted within 1 min.The relative standard deviations（RSDs） for nicotine and its two metabolites were between 0.29% and 4.0% and the recovery ranged from 94% to 104%.The linear correlations were above 0.998.Saliva,plasma and urine samples from 70 volunteers were collected and quantified by the described procedure.The classification of different smoking amount was also made using ada-boost based on the total concentration of salivary nicotine and its metabolites by a predicting accuracy of 76%.A good correlation of cotinine levels bewteen saliva and plasma（r=0.92）,saliva and urine（r=084） was found.The results showed that the cotinine in saliva has a longer half-life and becomes the most desirable biomarker for the evaluation of smoking exposure and nicotine uptake. |
| Close |