|
| Purification and Detection of Selenoprotein-P in Human Plasma by Affinity Chromatography-HG-AFS |
| |
| View Full Text Download reader |
| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:selenoprotein-P human plasma heparin-sepharose Ni-sepharose affinity chromatography HG-AFS |
| Author | Institution |
| 包建民，于晓燕，刘微，李优鑫 |
天津大学药物科学与技术学院天津市现代药物传递及功能高效化重点实验室 |
|
| Hits: 2643 |
| Download times: 1005 |
| Abstract: |
| A method was developed for the purification and detection of selenoprotein-P in human plasma.The sample was gradient eluted by two successive steps of affinity chromatography,i.e.,Heparin-Sepharose and Ni-Sepharose chromatography.The quality of the purified selenoprotein-P was detected by HG-AFS.The optimum gradient elution conditions were determined and the selenoprotein-P with a certain purity was obtained by SDS-PAGE.The recovery of the two steps of affinity chromatography reached up to 43.2%.The correlation coefficient of HG-AFS was 0.999 1，and the detection limit was 0.09 μg/L.The RSDs were 0.12% for within-day and 0.27% for inter-day.The recoveries were in the range of 95% - 104%.The proposed method was simple and sensitive,and the results obtained were accurate. |
| Close |