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| Ultrasensitive α-Fetoprotein Immunosensor Based on Electrochemical Stripping Analysis of Silver Deposition |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:α-fetoprotein silver deposition electrochemical stripping analysis immunosensor |
| Author | Institution |
| 王丽娟,贾翠娟 |
济宁职业技术学院生物与化学工程系 |
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| Abstract: |
| An electrochemical immunosensor was developed for the ultrasensitive measurement of α-fetoprotein based on electrochemical stripping analysis of silver nanoparticles catalytically deposited by nanogold labels.The immunosensor was prepared by covalently immobilizing capture antibodies with glutaraldehyde（GA） on chitosan multi wall carbon nanotubes modified glassy carbon electrodes.Through a sandwich type immunoreaction,antibody-functionalized AuNPs were captured onto the immunosensor surface,and further induced a subsequent silver deposition.The deposited AgNPs were then measured directly by anodic stripping analysis in KCl solution.Under the optimal conditions,the calibration curve was linear over α-fetoprotein concentration in the range of 0.02-200 μg/L,the linear regression equation is ΔI=-0.13-0.11c（μg/L）（r=0.992 8）.The detection limit is 8 ng/L.The electrochemical immunosensor was applied in the determination of α-fetoprotein in three serum samples.The relative standard deviation is no more than 6.1%.The determination result is identical with the result of electrogenerated chemiluminescence method.The sensor showed acceptable reproducibility,stability and accuracy,indicating a promising potential in clinical diagnose. |
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