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| Simultaneous Determination of Chlorogenic Acid and Phillyrin in ShuangHuangLian Oral Liquid and Capsule by HPLC with Online Solid Phase Extraction |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:online solid-phase extraction HPLC ShuangHuangLian oral liquid chlorogenic acid phillyrin |
| Author | Institution |
| 张艳海，张大伟，孟兆青，叶明立 |
1.戴安分析仪器有限公司;2.黑龙江省农垦总局总医院;3.江苏康缘药业股份有限公司;4.中国药科大学 |
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| Abstract: |
| An online SPE coupled with HPLC method was developed for the determination of chlorogenic acid and phillyrin in ShuangHuangLian oral liquid and capsule.Acclaim Polar AdvantageⅡC18（4.6 mm×50 mm×3 μm）was used as online one dimensional column with a mobile phase consisting of methanol-1% acetic acid-water by gradient elution with a right pump at a flow rate of 0.8 mL/min.The left pump was used to analyze the elute in SPE using Acclaim C18（4.6 mm×150 mm×5 μm）as its two dimensional analytical column by gradient elution with acetonitrile-1% acetic acid-water as mobile phase at a flow rate of 1.0 mL/min.The UV detector was set at 278 nm.Good linear relationships were observed between peak areas and concentrations in the ranges of 0.021-0.42 g/L for chlorogenic acid and 0.014-0.28 g/L for phillyrin，with correlation coefficients of 0.999 5 and 0.999 7，respectively.The average recoveries of phillyrin and chlorogenic acid in ShuangHuangLian oral liquid were 100% and 99%，respectively.This method was simple and fast,and had good speciality and repeatability. |
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