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| Determination of 3-Nitropropionic Acid in Zymin by Solid Phase Extraction Coupled with Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:solid phase extraction ultra performance liquid chromatography-tandem mass spectrometry 3-nitropropionic acid(3-NPA） zymin |
| Author | Institution |
| 张璐，乐爱山，郑玲，孔祥虹 |
1.陕西出入境检验检疫局;2.广西出入境检验检疫局 |
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| Abstract: |
| A method was developed for the quantitation of 3-nitropropionic acid in zymin by high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS）.The analyte in samples was extracted with acetonitrile and the extract was purified with PSA solid phase extraction column.The separation of 3-nitropropionic acid was carried out on a Waters HSS T3 column by gradient elution using acetonitrile-water as mobile phase.The mass analyzer was performed in negative MRM mode.The result showed that the calibration curve of 3-nitropropionic acid was linear in the range of 12.5-125 μg/L with correlation coefficients more than 0993.The limit of quantitation(LOQ） was 5.0 μg/kg.The recoveries of 3-nitropropionic acid at different spiked levels ranged from 69.1% to 114.4% with relative standard deviations(RSDs） of 0.9%-8.9%.The real sample test showed that the method was simple，rapid and reliable. |
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