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| Aptamer-based Chemiluminescent Detection of Adenosine |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:chemiluminescence adenosine aptamer magnetic bead horseradish peroxidase |
| Author | Institution |
| 鲁卢,严喜鸾,肖义陂,陈静雯,肖鉴谋 |
1.南昌大学环境与化学工程学院;2.南昌市洪都中医院 |
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| Abstract: |
| Based on magnetic beads as separating carrier and adenosine as a specific competitor appeared，a method of aptamer-based chemiluminescence(CL) for the determination of adenosine was developed.Experimental parameters including magnetic beads,adenosine-aptamer and report DNA were optimized.Under the optimal conditions，the decreased CL intensity was proportional to the adenosine concentration in the range of 1.0×10-9-1.0×10-4 mol/L(r2=0.99)，with a detection limit of 1.0×10-9 mol/L.The relative standard deviation(n=5) for 0.1 mmol/L adenosine is 5.0%.The present method was successfully applied in the determination of adenosine in real human urine.The method is simple，sensitive and specific for the detection of adenosine，and it is a promising approach for the determination of small biomolecules in the fields of clinical diagnosis，pharmaceutical analysis and so on. |
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