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| Rapid Determination of Illegally Added 25 Hormones in Oral by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:oral hormone ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS） |
| Author | Institution |
| HE Xiao-qin,XI Cun-xian,TANG Bo-bin,WANG Guo-min,PENG Tao,CHEN Dong-dong,MU Zhao-de* |
1.重庆医科大学药学院;2.重庆出入境检验检疫局重庆市进出口食品安全工程技术研究中心;3.中国检验检疫科学研究院 |
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| Abstract: |
| A sensitive ultra performance liquid chromatography-tandem mass spectrometric（UPLC-MS/MS） method was developed for the simultaneous determination of 9 progesterones,7 androgens,7 glucocorticoids,2 estrogens in anti-aging oral samples.The analytes were stepwise extracted with acetonitrile containing 1% acetic acid and methanol,the extract was concentrated to dryness,redissolved in methanol-water（1∶1）.25 hormones were gradient separated on a Shim-pack XR-ODS Ⅱ column,and analyzed by electrospray ionization under positive ion mode with matrix standard curve.As a result,the calibration curves for all target compounds were linear in the range of 0.5-50 μg/L with correlation coefficients larger than 0.99.The limits of detection（LOD） ranged from 0.029 μg/kg to 0.147 μg/kg and the limits of quantitation（LOQ） ranged from 0.099 μg/kg to 0.492 μg/kg.The average recoveries of 25 compounds at spiked levels of 0.5,1.0,5.0 μg/kg were in the range of 73.3%-122.2% with relative standard deviations（RSD） of 5.1%-14.9%.The method is simple,rapid and accurate,and is suitable for the rapid determination of hormones in anti-aging function foods. |
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