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| Determination of Streptomycin and Dihydrostreptomycin Residues in Eel and Honey by Liquid Chromatography-Tandem Mass Spectrometry |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:liquid chromatography-tandem mass spectrometry(LC-MS/MS) honey eel streptomycin dihydrostreptomycin residues |
| Author | Institution |
| ZHANG Wei ，SHENG Yong-gang，GU Shu-qing，DENG Xiao-jun*，CHEN Shun-sheng |
1.上海海洋大学食品学院;2.上海出入境检验检疫局 |
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| Abstract: |
| A liquid chromatography coupled with tandem mass spectrometric(LC-MS/MS) method was developed for the simultaneous determination of streptomycin and dihydrostreptomycin residues in eels and honey samples.The samples were extracted with sodium 1-heptanesulfonate and phosphate buffer,and cleaned up with a SUPELCO LC-C18 solid phase extraction(SPE) column.The extracts were separated on a Thermo Aquasil-C18 column(150 mm×4.6 mm,3 μm) using acetonitrile containing 0.3% acetic acid -0.3% acetic acid as mobile phase by gradient elution.The detection of streptomycin and dihydrostreptomycin was carried out by MS/MS under multiple reaction monitoring(MRM) mode.An external standard method was used for quantitative analysis.The method showed good linearities in the range of 10-100 μg/kg for streptomycin and dihydrostreptomycin,and the limits of quantitation(LOQ) were 10 μg/kg.The spiked recoveries of streptomycin and dihydrostreptomycin ranged from 89.6% to 110.3% with relative standard deviations of 2.3%-20.9%.The method was simple,rapid and practical,and could meet the requirements for determination of streptomycin and dihydrostreptomycin residues in eel and honey samples. |
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