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| Preparation and Application of Immunoaffinity Column for Ractopamine，Salbutamol and Clenbuterol |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:ractopamine salbutamol clenbuterol immunoaffinity-column high performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS) |
| Author | Institution |
| YU Le,HUO Guang-hua*,FU Jin-hua |
1.江西农业大学生物科学与工程学院，生物资源保护和利用研究所;2.江西省饲料监督监察所 |
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| Abstract: |
| The anti-ractopamine monoclonal antibody and anti-clenbuterol monoclonal antibody were both coupled with CNBr activated Sepharose 4B to make the immunoaffinity column.The column performance was tested and the use conditions were optimized by HPLC-MS/MS.The results showed that the column capacity to ractopamine,salbutamol and clenbuterol were 49,27,24 ng/mL glue,respectively,the storage time was five months,and the best eluent was methanol.The average recoveries of three lean meat essences ranged from 78.9% to 96.5% with relative standard deviations(RSD) lower than 1.5%.The limits of detection(LOD) ranged from 0.1 ng/g to 1 ng/g.The method could be applied in the simultaneous determination of the lean meat essence contents in feed samples. |
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