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| Separation and Characterization of Metallothionein-like Protein in Algae and Its Response to Zn and Cu Induced |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:metallothionein like protein heavy metal inductive effect mass spectrometric characterization algae |
| Author | Institution |
| LI Jing-xi*,GAO Li-jie，CAO Wei，CHEN Jun-hui，WANG Jiang-tao，ZHENG Li，WANG Xiao-ru |
1.国家海洋局第一海洋研究所海洋生态研究中心;2.中国海洋大学化学化工学院;3.中国地质大学（北京）海洋学院 |
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| Abstract: |
| With the kelp as a test vector,a new method of seperation and purification of metallothionein-like protein（MT-like） in algae was developed by using sephadex column.Then the MT-like protein and the Zn-MTs standard were measured by UV spectrophotometry.The molecular weight of MT-like protein were characterized by gel electrophoresis and accurately analysed by mass spectrometry.The enrichment ability of heavy metals was studied,and the response of MT-like to Zn and Cu was investigated after the kelp was cultured with ZnCl2,CuCl2,ZnCl2+CuCl2.The results showed that the MT-like and Zn-MTs had a similar UV absorption peak at 294 nm and displayed the similar range in SDS-PAGE gel electrophoresis.The molecular weight of the MT-like determined by electrospray ionization mass spectrometry was about 6.5 ku.The kelp showed a strong enrichment ability for metals,in which the enrichment ability for Zn was stronger than that for Cu.The Zn2+ could promote the MT-like production,while the growth of MT-like induced with Cu2+ increased at first,and then decreased.The Cu2+ had an antagonistic effect on Zn2+ inducing process. |
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