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| Detection of Carcinoembryonic Antigen(CEA) in Human Serum Using Label-free Amperometric Electrochemical Immunosensor |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:carcinoembryonic antigen K3［Fe(CN)6］-chitosan-glutaraldehyde conjugate label-free immunosensor cyclic voltammetry |
| Author | Institution |
| CHEN Min,WU Na-mei,CHEN Jin,LI Li-ying,ZHAO Cheng-fei,WENG Shao-huang,LIN Xin-hua |
1.福建医科大学药学院药物分析学系;2.福建医科大学附属协和医院骨科 |
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| Abstract: |
| A label-free immunosensor based on K3［Fe(CN)6］-chitosan-glutaraldehyde conjugate composite as signal indicator was developed for the easy and sensitive detection of carcinoembryonic antigen(CEA).The signal layer and nafion-modified glass carbon electrode(GCE) was used to wrap CEA antibodies.The morphology and electrochemical behaviors of the modified GCE substrates were characterized by atomic force microscopy(AFM) and cyclic voltammetry(CV),respectively.The result indicated that the response magnitudes of the anodic peak current were directly related to the concentration of CEA.A good linear relationship between decrease of current and logarithm of CEA concentration in the range of 0.005-40.0 ng/mL was obtained,with a detection limit of 1.23 pg/mL.The developed approach showed a high selectivity,and could be applied in the detection of CEA in human serum samples. |
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