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| Application and Development of Nanomaterials in Protein Phosphorylation Analysis |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:nanostructured materials protein phosphorylation enrichment of phosphopeptide signal amplification development |
| Author | Institution |
| WANG Zong-hua,SUN Na,LIU Yang,XIA Jian-fei,ZHANG Fei-fei,XIA Lin,XIA Yan-zhi |
1.青岛大学纤维新材料与现代纺织实验室国家重点实验室培育基地，化学科学与工程学院，山东省中日碳纳米材料合作研究中心，海洋生物质纤维材料及纺织品协同创新中心;2.清华大学化学系 |
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| Abstract: |
| Protein phosphorylation is one of the most important post-translational modifications,and also plays a vital role in cellular metabolism.Aberrant protein phosphorylation states and kinase activity are associated with many human diseases such as cancer,diabetes mellitus and heart disease.Due to the dynamic reversibility of protein phosphorylation and the low abundance of phosphoprotein/phosphopeptide in biological samples,it is a big challenge to the ultrasensitive detection of protein phosphorylation,which is most significant for earlier clinical analysis on disease and drug screening.In this work,the mechanism for selective recognization or capture of phosphoprotein/phosphopeptide was discussed.The main mechanisms for enrichment of phosphoprotein/phosphopeptide or signal amplification in protein phosphorylation analysis based on nanostructured materials were introduced in detail.Based on that mechanism,the recent application and development of nanostructured materials in protein phosphorylation analysis were reviewed. |
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