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| Determination of Heliotrine in Cynoglossum amabile Stapf et Drumm by HPLC with Focused Microwave-assisted Extraction |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:focused microwave-assisted extraction Cynoglossum amabile Stapf et Drumm heliotrine high performance liquid chromatography(HPLC) |
| Author | Institution |
| FAN Qing-ya，WU Chang-zhong，WANG Wan-hao |
1.信阳职业技术学院药学院;2.河南同源制药有限公司 |
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| Hits: 2016 |
| Download times: 930 |
| Abstract: |
| An extraction method of heliotrine was developed by focused microwave-assisted extraction(FMAE).Orthogonal design was used to optimize the experimental conditions.The optimum extraction conditions were as follows: 60 mesh size of material，solvent-materail ratio:1∶35，microwave power:420 W and extraction time:50 min.The analyte was determined by HPLC using methanol -0.05%triethylamine(pH 7.5 )(30∶70) as mobile phase with a detection wavelength of 220 nm.The linear range of heliotrine was in the range of 52-1 040 mg/L(r=0.999 9)，with a detection limit of 0.040 2 mg/L.The recoveries for heliotrine were in the range of 97.5%-103%with RSDs of 2.4%.The method was simple and highly sensitive. |
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