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| Investigation on Determination of T-2 Toxin in Cereal and Feed by Immunoaffinity Column Purification/LC-MS/MS |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:T-2 toxin immunoaffinity column LC-MS/MS quantitative analysis cereals and feed |
| Author | Institution |
| LI Jing，MA Fei，LI Pei-wu，ZHANG Qi，DING Xiao-xia，ZHANG Wen |
1.中国农业科学院油料作物研究所;2.农业部生物毒素检测重点实验室;3.农业部油料作物生物学与遗传育种重点实验室;4.农业部油料产品质量安全风险评估实验室（武汉）;5.农业部油料及制品质量监督检验测试中心 |
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| Abstract: |
| Immunoaffinity column（IAC） for T-2 toxin was prepared with anti-T-2 toxin monoclonal antibody 2G7 and CNBr activated Sepharose 4B.The experimental conditions for T-2 immunoaffinity column were optimized and the columns were applied in cereals and feed pretreatment.An IAC/LC-MS/MS method was established for the determination of T-2 toxin in cereals and feed.The calibration curve for T-2 toxin standard solution was linear in the range of 0.5-500.0 ng/g with correlation coefficient of 0.999 7.The limit of detection（LOD） and limit of quantitation（LOQ） was 0.05 ng/g and 0.17 ng/g，respectively.The average recoveries of T-2 toxin in rice，maize and feed samples at three spiked levels of 10，50，100 ng/g ranged from 92.9% to 109.7%，with relative standard deviations（RSDs） of 2.1%-8.3%.The method was applied in the detection of T-2 toxin in 36 real samples with RSDs less than 10.0%. |
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