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| Determination of Hemoglobin in Single Human Red Blood Cell by Microchip Electrophoresis with Chemiluminescence Detection |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:microchip electrophoresis chemiluminescence detection single cell analysis human red blood cells hemoglobin |
| Author | Institution |
| LI Xiang-tang,ZHANG Li-chun,ZHAO Shu-lin* |
广西师范大学化学与药学学院 |
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| Abstract: |
| In this paper,an application of microchip electrophoresis with chemiluminescence detection（MCE-CL） in single human red blood cell analysis was described.Human red blood cells were assayed to determine the intracellular content of hemoglobin（Hb）.The injection of individual cells,in-line lysed,and MCE separation were integrated onto a simple cross microfluidic chip.CL detection was based on the catalytic effects of hemoglobin on the luminol-hydrogen peroxide reaction.The MCE-CL detection had a linear calibration curve over the range of 2.0-90 pg injected with a correlation coefficient of 0.998 9 and a detection limit（S/N=3） of 0.8 pg.The content of Hb in individual human red blood cell was found in the range of 14-68 pg（n=19）.Compared to other methods,the MCE-CL method is simple and sensitive,and could be an attractive alternative for single cell analysis. |
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