|
| Determination of Six Active Components in Chuanbei Pipa Syrups by Micro-fluidic Evaporative Light Scattering Detector Coupled to Pressurized Capillary Electrochromatography |
| |
| View Full Text Download reader |
| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:microfluidic evaporative light scattering detector（μELSD） pressurized capillary electrochromatography（pCEC） fritillaria alkaloids platycodin D oleanolic acid |
| Author | Institution |
| ZHOU Wen-li,KAN Wen-bin,WANG Yu-hong,ZHANG Lin,WANG Yan,YAN Chao* |
1.上海交通大学药学院;2.上海通微分析技术有限公司 |
|
| Hits: 2301 |
| Download times: 470 |
| Abstract: |
| Microfluidic evaporative light scattering detector（μELSD） coupled to pressurized capillary electrochromatography（pCEC） was applied in the separation and detection of six active components,e.g.platycodin.D,peimisine,oleanolic acid,imperialine,peimine and peiminine in Chuanbei Pipa Syrups.The simultaneous analysis of six active components by pCEC-μELSD was performed on a C18 capillary column（EP-150-15-3-C18） with mobile phase A:acetonitrile and mobile phase B:10 mmol?L-1 formic acid-triethylamine aqueous solution（pH 11.0） by gradient elution.The optimal conditions were as follows:column flow rate:1.02 μL?min-1,applied voltage:+10 kV,nebulizing gas flow rate:0.4 L?min-1 and evaporative temperature:40 ℃.Six active components in Chuanbei Pipa Syrups were successfully separated in 10 min with the excellent linearity of four orders of magnitude.The limit of detection（LOD） could reach up to the level of pg,and the recoveries were between 97.9% and 103.0%.The precision,repeatability and stability indicated a good reproducibility for the determination.The results also demonstrated the feasibility and practicality of pCEC-μELSD platform.The method could be used for the determination of the active ingredients in Chuanbei Pipa Syrups. |
| Close |
|
|
|