|
| Quantitative Analysis of 18 Active Components in Bansha Herbal Tea by Ultra High Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry |
| |
| View Full Text Download reader |
| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:ultra high performance liquid chromatography coupled with tandem mass spectrometry（UHPLC-MS/MS） Bansha herbal tea（BHT） active components quantitative analysis |
| Author | Institution |
| LIANG Hui,ZHU Wei-jian,YUAN Zhi-quan,CHEN Chao,WU Qing-hui,YANG Yun-yun |
1.中国广州分析测试中心广东省化学危害应急检测技术重点实验室;2.安捷伦科技有限公司安捷伦-中广测联合技术中心 |
|
| Hits: 2641 |
| Download times: 498 |
| Abstract: |
| A method for the quantitative analysis of 18 active components in Bansha herbal tea（BHT） by ultra high performance liquid chromatography coupled with tandem mass spectrometry（UHPLC-MS/MS） was developed.BHT samples were extracted with methanol,and the extracts were separated on an RRHD SB-C18 column with 0.5% formic acid aqueous solution-acetonitrile as mobile phase by gradient elution.Quantitative analysis was performed by a triple quadrupole MS system operated under multiple-reaction monitoring（MRM） mode in negative ion detection mode.The method showed a good linearity with correlation coefficients（r） not less than 0.996 4 for 18 investigated analytes.The limits of detection and quantitation were in the ranges of 0.001-0.08 μg/mL and 0.002-0.27 μg/mL,respectively.In addition,high precision,good accuracy,and desirable stability were also obtained.The developed method was then used for the determination of the contents of 18 active components in 11 Huangzhenlong（HZL） and 8 Pingantang（PAT） BHT samples.The results showed that the concentrations of 18 investigated active components ranged from 0.7 to 149.8 μg/mL and 0.9 to 110.3 μg/mL for HZL and PAT samples,respectively.Principal component analysis（PCA） was performed to evaluate the quality stability and consistency and trace the origins of the 19 batches of investigated BHT samples.PCA results showed that two manufactures BHT samples were easy to differentiate by using the PCA score plot,indicating that the formulas and the production processes of the two BHT products were different.The proposed method was a desirable approach for the quality assessment and control of BHT. |
| Close |
|
|
|