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| Detection of Single DNA Molecule with Magnetic Microsphere Probe Based on Volume Amplification |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:magnetic sphere volume amplification DNA single molecule detection |
| Author | Institution |
| LI Lin-lin，XU Shi-ming，LIU Peng-fei,WANG Tian,ZHANG Xiao-li |
1.山东省产品质量检验研究院;2.山东省材料化学安全检测技术重点实验室;3.山东大学化学与化工学院 |
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| Abstract: |
| In this paper,a novel method to detect single DNA molecule was proposed.A sandwich-type hybridization assay was performed on a streptavidin-coated substrate.A large number of biotinylated capture DNAs（c-DNAs） were first covalently bound to the substrate through the interaction between streptavidin and biotin.Then,the complementary target DNAs（t-DNAs） were hybridized with the c-DNAs attached to the substrate,followed by hybridization between t-DNAs and biotinylated first probe DNAs（p-DNAs） conjugated to streptavidin coated magnetic microparticles（MMPs）.In this case,one t-DNA binding one MMP could be observed with an optical microscope.The number of the MMPs is linearly proportional to concentration of t-DNA in the range of 5×10-16-1×10-14 mol/L.This size amplification method was simple and could be applied in other biomolecules. |
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