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| Preparation of Ag@SiO2/ZnO@SiO2 and Its Application in Detection of Biological Molecules in Aqueous Solution |
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| DOI： |
| KeyWord:zinc oxide nano silver fluorescence stability analysis of test hemoglobin L-cysteine |
| Author | Institution |
| HUANG Wen-yi，WEI Hui-dan，SHI Zhi-peng，BAI Da-wei，MA Lan-yu,LI Li-jun* |
广西科技大学生物与化学工程学院，广西糖资源绿色加工重点实验室，广西高校糖资源
加工重点实验室 |
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| Abstract: |
| A simple，rapid and mild condition method was developed for the preparation of ZnO@SiO2 core-shell structures quantum dots and Ag@SiO2/ZnO@SiO2 nanocomposite.Particle sizes，UV-vis absorption and emission spectra were characterized by transmission electron microscopy（TEM），ultraviolet spectrometry（UV），fluorescence spectroscopy（FL），nanometer particle size analyzer and ZETA potentials techniques.The results showed that the particles sizes of ZnO@SiO2 core-shell structures quantum dots were 3-8 nm and could emit yellow green fluorescence under the excitation of UV-vis light.Their maximum absorption wavelength was 330 nm，and the maximum fluorescence emission wavelengths were located at 510 nm.Ag@SiO2/ZnO@SiO2 could enhance the fluorescent of ZnO@SiO2 core-shell quantum dot，but it didn-t - change the emission peak position of ZnO@SiO2 core-shell quantum dot.Otherwise，Ag@SiO2/ZnO@SiO2 greatly enhanced the fluorescent stability of ZnO@SiO2 core-shell quantum dots at room temperature.Based on different level quenching of hemoglobin and L-cysteine for quantum dots,a simple，rapid and sensitive detection method was established.Under the optimized conditions，the characteristic fluorescence intensity of Ag@SiO2/ZnO@SiO2 at 510 nm was linearly proportional to the concentration of L-cysteine and hemoglobin in ranges of 0.068-10.10 mg/L and 4.00×102-4.00×103 mg/L，respectively.The detection limits were 6.92×10-3 mg/L and 2.04 mg/L，respectively，and the correlation coefficients were 0.993 8 and 0.995 1,respectively.The relative standard deviations for 11 replicate detections of 3.37 mg/L L-cysteine and 2.00×103 mg/L hemoglobin were 1.3% and 2.5%，respectively. |
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