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| Determination of Solvent Yellow 2 and Solvent Yellow 56 in Dried Bean Curd and Yuba by Liquid-Liquid Extraction/High Performance Liquid Chromatography |
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| DOI： |
| KeyWord:liquid-liquid extraction high performance liquid chromatography(HPLC) dried bean curd yuba solvent yellow 2 solvent yellow 56 |
| Author | Institution |
| WANG Hui*，LI Yong-qiang，PENG Xin-kai，XIA Li-xin，OUYANG li，ZENG Ling-yi |
1.长沙市食品质量安全监督检测中心;2.湖南省食品安全生产工程技术研究中心;3.常德市产商品质量监督检验所 |
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| Abstract: |
| A method for the determination solvent yellow 2 and solvent yellow 56 in dried bean curd and yuba was developed using liquid-liquid extraction-high performance liquid chromatography(LLE-HPLC).The samples were extracted by ultrasonic using hexane saturated with acetonitrile.Then，the target compounds were vortex extracted using acetonitrile saturated with hexane.The acetonitrile was moved into concentrated bottle.Operation steps were repeated for three times.The extraction solution was kept in constant volume after concentration.The concentrated solution was tested by high performance liquid chromatography.The target compounds were separated on a TC-C18(4.6 mm×250 mm，5 μm) column using water-methanol(20∶80) as mobile phase at a flow rate of 1.0 mL/min. The column temperature was set at 30 ℃,and the detection wavelength was 410 nm.Under the optimal conditions，the calibration curves of solvent yellow 2 and solvent yellow 56 were linear in the range of 0.30-50 mg/L.The limits of detection(LOD) of two targets compounds were both 0.15 mg/kg,and the limits of quantitation(LOQ) were both 0.50 mg/kg.The average spiked recoveries of two target compounds in dried bean curd and yuba were in the range of 85.4%-94.6%，with relative standard deviations(RSD，n=6) of 0.3%-2.3%.With good repeatability and high sensitivity，the method was suitable for the determination solvent yellow 2 and solvent 56 in dried bean curd and yuba samples. |
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