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| Magnetic Affinity Immunoassay Based on Gold-magnetic Particle and HRP-conjugated Phage Antibody |
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| DOI： |
| KeyWord:gold-magnetic particle functionalized probe HRP-conjugated phage antibody abrin detection |
| Author | Institution |
| MU Xi-hui,TONG Zhao-yang*,HUANG Qi-bin,LIU Bing,LIU Zhi-wei,HAO Lan-qun,ZHANG Jin-ping |
国民核生化灾害防护国家重点实验室 |
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| Abstract: |
| A new magnetic affinity immunoassay（MAIA） method based on gold-magnetic particle and HRP-conjugated phage antibody was developed.Gold-magnetic particle were coated with staphylococcus protein A（SPA） and coupled with polyclonal antibody（pcAb） to construct functionalized capturing probes,and HRP-conjugated phage antibody was then used as specific detecting probe to design MAIA for trace detection of abrin.The calibration curve was linear（r=0.991 0） in the range of 0.008-250 μg/L,with a limit of detection（LOD） of 0.008 μg/L,and a limit of quantitation（LOQ） of 0.008 μg/L.Integrating the unique advantages of functionalized SPA gold-magnetic particle probe and HRP-conjugated phage antibody with its high sensitivity,specificity and anti-interference,the method is proven to be well suited for the analysis of trace abrin in various environmental samples. |
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