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| Determination of L-Carnitine in Dairy Products by High Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry |
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| DOI： |
| KeyWord:HPLC-MS/MS L-carnitine dairy products internal standard |
| Author | Institution |
| XU Dun-ming*,CHEN Yan,ZHANG Jin,YU Yu-cheng,FENG Feng,ZHANG Feng,CHU Xiao-gang |
1.厦门出入境检验检疫局检验检疫技术中心;2.中国检验检疫科学研究院食品安全研究所 |
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| Abstract: |
| A method of HPLC-MS/MS with internal standard for the determination of L-carnitine in dairy products was developed.After the sample was dissolved in 0.1 mol/L HCl solution and its protein was precipitated with acetonitrile,the object was separated with a HILIC chromatographic column,tested by HPLC-MS/MS with sheath flow electro-spray ionization under positive ion scanning and multiple reaction monitoring(MRM) mode,and quantified by the internal standard method.The average recoveries at three spiked levels were in the range of 85.2%-102.0% with RSDs(n=6) of 7.6%-14.3%.The limit of detection (LOD) was 50 μg/kg and the limit of quantitation(LOQ) was 200 μg/kg.The proposed method was applied in the analysis of several real samples of different origins from overseas,and 20 samples were screened for L-carnitine with content range of 68-165 mg/kg,of which only 1 sample did not match with the label.This method was rapid,simple and accurate,and was suitable for the determining of L-carnitine content in dairy products. |
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