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| Identification of Four Active Constituents in Hair Growth Cosmetics by Ultra Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry |
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| DOI： |
| KeyWord:solid phase extraction(SPE) ultra performance liquid chromatography quadrupole-time-of-flight mass spectrometry(UPLC-QTOF-MS) hair growth cosmetics active constituents |
| Author | Institution |
| XIE Wen-jian,XIONG Xiao-ting,XI Shao-feng,LI Hui-yong,TAN Jian-hua*,WANG Ji-cai,ZHAO Tian-tian,GUO Chang-hong |
1.广州质量监督检测研究院国家化妆品质量监督检验中心广州;2.华南农业大学资源环境学院 |
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| Abstract: |
| A method for the determination of four active constituents(i.e. 2,3,5,4′-tetrahydroxy stilbene-2-Ο-β-D-glucoside(THS-GLU), quercetin-3-rhamnoside(QR), rutin(RT)and naringin(NG))in hair growth cosmetics was developed by strong anion exchange-solid phase extraction(SPE) combined with ultra performance liquid chromatography quadrupole-time-of-flight mass spectrometry(UPLC-QTOF-MS).The sample was ultrasonically extracted with methanol,and purified with SPE(PAX,500mg/6mL) cartridge.Chromatographic separation was conducted on a UPLC CSH C18(2.1mm×50mm,1.7μm,Waters) column by gradient elution using methanol and 0.002% formic acid solution as mobile phases.The MS analysis was carried out in electrospray ionization operated in negative mode.THS-GLU,QR and RT were quantified by the external standard method.NG,which arised configuration conversion to naringin chalcone,was just identified by qualitative analysis.Under the optimal conditions, satisfactory linearities(r2>0.999) for three compounds were obtained for all analytes in the concentration range of 5-200μg/L.Limits of quantitation(S/N=10) were in the range of 0.03-0.1mg?kg-1.Recoveries at three spiked levels were in the range of 80.9%-104.7% with relative standard deviations(RSD,n=6) not more than 19.6%.This method was accurate and applicable,and was succcessfully applied in the determination of THS-GLU,QR,RT and the identification of NG in hair growth cosmetics. |
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