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| Spectrofluorimetric Determination of Indole Phytohormones in Bean Sprout Using Three Phase Hollow Fiber-Liquid Phase Microextraction |
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| DOI： |
| KeyWord:hollow fiber three phase liquid phase microextraction indole phytohormones orthogonal experiment spectrofluorimetry bean sprout |
| Author | Institution |
| CHEN Lu, TURGHUN Muhammad*, MUNIRA Abuduani, SHIRALI Jappar |
新疆大学化学化工学院石油天然气重点实验室 |
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| Abstract: |
| A new method using three phase hollow fiber-liquid phase microextraction was developed for the determination of indole phytohormones in bean sprout by spectrofluorimetry.The parameters affecting liquid phase microextraction performance were optimized using L9(34)orthogonal experiment.The optimum extraction was achieved by adjusting sample solution pH value to 4.0, using 1-octanol as organic extraction solvent, pH 12.0 solution as acceptor phase, and stirring the solution at 1 000 r/min for 60 min.Under the optimum conditions, the calibration curve showed a good linearity(r=0.997 9) in the range of 1.71-50.0 mg/L, with a detection limit of 0.57 mg/L.Recoveries for spiked samples from 88.6%to 100.7%were obtained with RSDs not more than 4.8%.The proposed method was simple, green and accurate in the determination of indole phytohormones in bean sprout. |
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