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| A Miniaturized Aflatoxin Analyzer Based on LED induced Fluorescence Detector |
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| DOI： |
| KeyWord:aflatoxin fluorescence light emitting diode analyzer |
| Author | Institution |
| XU Jing*,ZHANG Ning,TIAN Hong-zhe,WANG Dan ,LI Yan ,LIU Hui-ying,CAO Ji-juan* |
1.辽宁出入境检验检疫局国家食品安全检测重点实验室;2.沈阳农业大学分析测试中心 |
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| Abstract: |
| In the present method for aflatoxin detection,the low-end devices could not be applied in quantitation of multi-components,while the high-end devices are hard to be used in small labs due to the high cost and large volume.In this situation,a miniaturized aflatoxin analyzer was constructed based on micellar electrokinetic capillary chromatography with light emitting diode（LED） induced fluorescence detector.The main parts of the apparatus were chosen as follows:A UV LED was used as the light source,two band pass filters including BP 365 and BP 430 were used for the excitation light and the fluorescence,respectively,ultra violet hard clad silica(UVHCS) fiber was used to direct the fluorescence,and photomultiplier tube（PMT） was used as the detection element.The buffer composition was investigated and the optimized conditions were found.Under the optimal conditions,baseline separation of six aflatoxins was achieved.Good linearities were obtained over the concentration range of 0.1-10 μg/L for six compounds with correlation coefficients larger than 0.99.The intra-RSDs were in the range of 2.0%-2.4%,while the inter-RSDs were 5.1%-6.0%,respectively.The limits of detection for six compounds were in the range of 0.3-0.8 μg/kg,with recoveries of 85.3%-97.0%.With the advantages of small volume and low cost,as well as the elimination of derivatization and organic reagent,this analyzer could be widely used for the multi-aflatoxin quantitation in food analysis. |
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