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| Determination of Vitamin D2 and Vitamin D3 in Dairy Products by HPLC-APCI(+)-MS/ MS |
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| DOI： |
| KeyWord:high performance liquid chromatography-triple quadrupole mass spectrometry(HPLC-MS/MS) atmospheric pressure chemical ionization mass spectrometry vitamin D2 vitamin D3 dairy products |
| Author | Institution |
| HUO Yan-min*,WANG Jun,DUAN Wen-zeng*,XUE Xia,WANG Yan-li,YU Wen-jiang |
1.聊城大学化学化工学院;2.山东省食品药品检验研究院食品检验室;3.泰山学院化学化工学院 |
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| Abstract: |
| An HPLC-APCI(+)-MS-MS method was developed for the determination of vitamin D2(VD2) and vitamin D3(VD3) in dairy products.The sample was saponified,and then extracted with petroleum ether.Next,the extract was dried with anhydrous sodium sulfate and concentrated below 10 mL via rotary evaporation under the protection of nitrogen.Then it was dried with nitrogen and dissolved with 10 mL methanol.The extract was analyzed by HPLC-APCI(+)-MS-MS using an ACQUITY UPLC BEH C18 column(1.7 μm,2.1 mm×100 mm) as chromatographic column with a mobile phase of methanol and water under gradient elution.The responses of VD2 and VD3 showed good linearities in the range of 0.01-0.2 mg/L,with correlation coefficients more than 0.998. The average recoveries were in the range of 83.0%- 99.4%,with RSDs of 2.0%- 8.3%. |
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