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| Rapid Determination of Clindamycin Hydrochloride by Contactless Conductivity Detection with Microfluidic Chip |
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| DOI： |
| KeyWord:microfluidic chip contactless conductivity detection clindamycin hydrochloride capsules clindamycin hydrochloride |
| Author | Institution |
| TONG Yan-li,ZHAI Hai-yun*,LIU Cui,MEI Hua-qing,PAN Yu-fang,CHEN Zuan-guang |
1.广东省第二人民医院;2.广东药科大学药科学院;3.中山大学药学院 |
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| Abstract: |
| A method for the rapid determination of clindamycin hydrochloride in clindamycin hydrochloride capsules by microchip capillary electrophoresis with contactless conductivity detection was developed.Influence parameters on the separation and detection such as type and concentration of buffer,type of additive,separation voltage and injection time were investigated.Under the optimal conditions,using 2.0 mmol?L-1 HAc-2.0 mmol?L-1 NaAc as buffer solution,clindamycin hydrochloride was separated and detected within 1.0 min at a separation voltage of 1.5 kV and an injection time of 15. 0 s.Clindamycin hydrochloride showed a good linear response in the range of 20.0-200.0 μg?mL-1,with a correlation coefficient（r） of 0.999 2.The limit of detection（S/N=3）was 5.0 μg?mL-1 with RSD of 21%,and the recoveries were in the range of 95.9%-103%.This method was simple and rapid,and could be used for the detection and quality control of clindamycin hydrochloride in clindamycin hydrochloride capsules. |
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