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| Research on Development of Dopamine Fluorescence Detection Method Based on CdSe/ZnS Quantum Dots |
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| DOI： |
| KeyWord:CdSe/ZnS quantum dots dopamine fluorescence detection |
| Author | Institution |
| SHEN Chen-fan,ZHANG Zhi-feng,GUO Yu-an,YAN Gui-qin* |
山西师范大学生命科学学院 |
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| Abstract: |
| By taking CdSe/ZnS quantum dots as a fluorescence probe,a method for the rapid detection of fluorescence of dopamine was developed based on fluorescence quenching effect of dopamine to CdSe/ZnS quantum dots.Under the optimal experimental conditions(pH 7.4,reaction time:20 min),the fluorescence quenching strength ratio of CdSe/ZnS quantum dots had a good linear relationship(r=0.996) with concentration of dopamine in the range of 0.01-0.7 μmol/L.The detection limit was 1.6×10-4 μmol/L,and the relative standard deviation of this method was 1.2%.Compared with some approaches reported in current literatures,this method has a lower detection limit and higher sensitivity.The method could be applied in the rapid detection of dopamine injection and dopamine in human’s urine sample. |
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