|
| Determination of Azithromycin in Formula Feed for Fattening Pigs by High Performance Liquid Chromatography-Tandem Mass Spectrometry |
| |
| View Full Text Download reader |
| DOI： |
| KeyWord:liquid chromatography tandem mass spectrometry（LC-MS/MS） azithromycin fattening pig formula feed |
| Author | Institution |
| TANG Xiang，ZHAO Li-jun，LUO Cheng,WU Cai-mei*，YAN Shan-shan，LIU Guang-mang，LIN Yan，WU De |
1.四川农业大学动物营养研究所，四川省动物抗病营养重点实验室，农业部动物抗病营养与饲料重点实验室;2.农业部畜禽产品质量安全风险评估实验室（成都）;3.四川大学化学工程学院 |
|
| Hits: 2088 |
| Download times: 946 |
| Abstract: |
| A method was developed for the determination of azithromycin in formula feed for fattening pigs by liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）.The sample was extracted with acetonitrile under ultrasonic assistance.Oasis MCX solid phase extraction column was used for purification，and the chromatographic separation was performed on a Hypersil BDS C18 column（2.4 μm，100 mm×2.1 mm） with gradient elution using 0.05 mol/L ammonium acetate-methanol（1∶9） as mobile phase.The detection of azithromycin was performed with positive ion under multiple reaction monitoring（MRM） mode.The internal standard isotope dilution method was used for quantification.A good linearity（r2=0.999 1） was obtained for azithromycin in the range of 1-250 μg/L.The limit of detection（LOD，S/N≥3） and the limit of quantitation（LOQ，S/N≥10） of azithromycin in the formula feed were 2.8 μg/kg and 8.4 μg/kg，respectively.Average recoveries ranged from 87.0% to 106.6% for the formula feed at spike levels of 0.5，1，5，10 mg/kg.The intra-RSDs for azithromycin were 0.58%-5.4% and the inter-RSDs were 3.1%-5.4%.The method is accurate and sensitive，and the matrix interference is small.The method could be applied in the determination of azithromycin in formula feed. |
| Close |