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| Determination of the Main Anthraquinones in Root of Polygonum Cuspidatum by Pressurized Capillary Electrochromatography |
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| DOI： |
| KeyWord:pressurized capillary electrochromatography polygonum cuspidatum anthraquinone |
| Author | Institution |
| XIAO Xiao-ru，FENG Jun，CHENG Hao，HUANG Wen-yi，LI Yan-qing，ZHAO Yan-yong，LI Li-jun* |
1.广西科技大学生物与化学工程学院;2.广西科技大学，广西糖资源绿色加工重点实验室;3.广西科技大学，广西高校糖资源加工重点实验室;4.广西科技大学医学院药学系 |
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| Abstract: |
| A pressurized capillary electrochromatographic（pCEC） method was developed for the determination of the main anthraquinones,including rhein，emodin，aloe emodin，chrysophanol and physcion in root of Polygonum cuspidatum.The separation was performed on a reversed phase EP-100-20/45-3-C18 column（total length of 45 cm,effective length of 20 cm，diameter of 100 μm，ODS packing inside for 3 μm）.The mobile phase was composed of 20 mmol/L NaH2PO4（pH 4.7）-acetonitrile（15∶85） at a flow rate of 0.04 mL/min.Under the optimum conditions including running voltage of +5 kV，UV detection wavelength of 254 nm，the limits of detection（S/N=3）were in the range of 0.60-2.54 μg/mL for rhein，aloe emodin，emodin，chrysophanol and physcion，respectively,and the linear detection ranges were 3.57-162.68 μg/mL with correlation coefficients not less than 0.998 2.The established method was used in the analysis of anthraquinones in polygonum cuspidatum with good results.The recoveries at three spiked levels ranged from 91.1% to 101.2%，with relative standard deviations（RSD） of 0.03%-3.6%. |
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