| This work describes the development of a sensitive and reliable method using QuEChERS sample preparation and high performance liquid chromatography-diode array detector(HPLC-DAD) for simultaneous determination of phytohormones, zeatin(Z), gibberellic acid(GA), paclobutrazol(PBZ), 4-fluorophenoxyacetic acid(4-FPA), 4-chlorophenoxyacetic acid(4-CPA), 3-indolyl-acetic acid(IAA), 3-indolyl-butyric acid(IBA), 6-benzylaminopurine(6-BA), abscisic acid(ABA), 1-naphthyl acetic acid(NAA), forchlorfenuron(CPPU), 2,4-dichlorophenoxyaceticacid(2,4-D), 2,4,5-trichlorophenoxyacetic acid(2,4,5-T), in fruits and vegetables.The samples were extracted with 80% acetonitrile containing 0.5% acetic acid,and the extract was cleaned up with dispersive solid phase extraction adsorbent(powder of C18 and diatomaceous earth).The phytohormones were separated on a Waters XBridge C18,and eluated utilizing a gradient elution program of acetonitrile and water containing 0.05% acetic acid(by volume) as mobile phase.The correlation coefficients of calibration curves were over 0.99 in the corresponding concentration ranges.The average recoveries of the 13 analytes at three spiked concentration levels varied from 68.4% to 95.1% with relative standard deviations(RSD) less than 5%.The limits of detection(LOD) and quantitation(LOQ) were in the ranges of 0.005-0.020 mg/kg and 0.01-0.09 mg/kg,respectively.The real sample tests showed that the proposed method was simple,rapid,reliable and cost-effective,and was suitable for the simultaneous determination of phytohormones in fruits and vegetables. |