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| Determination of Quinolones in Hotpot Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry |
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| DOI： |
| KeyWord:SPE ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) quinolones hotpot |
| Author | Institution |
| ZHAO Fei,GAO Guang-hui,JIA Hong-xin |
辽宁省食品检验检测院 |
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| Abstract: |
| An ultra performance liquid chromatography-tandem mass spectrometric(UPLC-MS/MS) method was established for the determination of 14 quinolones in hotpot.Samples were extracted with acidified acetonitrile,defated with n hexane,separated on a C18 solid phase extraction(SPE) column,and analyzed by LC-ESI-MS/MS under the positive mode with multiple reaction monitoring(MRM).The method showed good linearities for 14 quinolones over the range of 7.5-100 μg/L with correlation coefficients(r) more than 0.990.The detection limits of 14 quinolones were 0.5 μg/kg,and the quantitation limits were 1.5 μg/kg.The recoveries of all analytes in hotpot ranged from 75.4% to 110.0%with relative standard deviations(RSD,n=6)of 1.3%-10.1%.The method has the advantages of simplicity,rapidness,good purifying effect and sensitivity,and is suitable for the simultaneous determination of quinolones in hotpot. |
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