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| Determination of Malachite Green，Crystal Violet and Their Metabolites Residues in Aquatic Product by QuEChERS Combined with UPLC-MS/MS |
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| DOI： |
| KeyWord:QuEChERS EMR-Lipid UPLC-MS-MS malachite green(MG) leucomalachite green(LMG) crystal violet(CV) leucocrystal violet(LCV) |
| Author | Institution |
| GONG Xiao-ming，HUA Meng-meng，WANG Hong-tao,WANG Bing-jun,MA Rong-hui |
潍坊出入境检验检疫局 |
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| Abstract: |
| A method for the determination of malachite green(MG)，crystal violet(CV) and their metabolites residues leucomalachite green(LMG) and leucocrystal violet(LCV) in aquatic product was developed using QuEChERS combined with UPLC-MS-MS.The sample was extracted with acetonitrile and purified by EMR-Lipid.The target compounds were separated on an Agilent Eclipse Plus C18(1.8 μm,3.0 mm×100 mm) column,and detected by tandem mass spectrometry with electrospray ionization in positive mode.The linear ranges were in the range of 0.2-10.0 μg/L and the correlation coefficients were all above 0.997．The average recoveries of 4 analytes in fish matrix at three spiked concentrations of 0.5，1.0，5.0 μg/kg ranged from 77.1% to 106.6%,with relative standard deviations of 1.3%-4.3%．This method was simple and reliable，and could effectively remove the protein and fat in sample.Therefore,it is suitable for the determination of MG,CV and their leuco metabolites residues in aquatic product. |
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