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| Determination of 1-(2-Hydroxyethyl)piperidine in Flavoxate Hydrochloride by HPLC-QTRAP-MS/MS |
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| DOI： |
| KeyWord:high performance liquid chromatography-quadrupole ion trap tandem mass spectrometry(HPLC-QTRAP-MS/MS) flavoxate hydrochloride 1-(2-hydroxyethyl)piperidine |
| Author | Institution |
| XU Jie,ZHOU Chang-peng,ZHENG Wen-feng,WANG Chun-ling |
迪沙药业集团国家认定企业技术中心 |
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| Abstract: |
| A high performance liquid chromatography-quadrupole ion trap tandem mass spectrometric(HPLC-QTRAP-MS/MS) method was developed for the quantification of 1-(2-hydroxyethyl) piperidine residue in flavoxate hydrochloride.After dissolved with 0.1% formic acid,the flavoxate hydrochloride sample was loaded onto an Agilent ZORBAX SB-Aq C18(2.1 mm×100 mm，1.8 μm) column to separate with a mobile phase of 0.1% formic acid-acetonitrile.The electrospray was operated in positive ion mode,and the sample was monitored in the select reaction monitoring(SRM) mode.As a result,the calibration curve of 1-(2-hydroxyethyl)piperidine showed a good linearity(r=0.999 7) in the range of 10-100 μg/L.The recoveries were in the range of 98.9%-106.8%,with relative standard deviations(RSDs) not more than 3.0%.The limit of detection(LOD,S/N=3) for the method was 6.0 mg/L,and the limit of quantitation(LOQ,S/N=10) was 20 mg/L.The method has the advantages of simple operation,high sensitivity and good reproducibility,and could be used for the detection of 1-(2-hydroxyethyl)piperidine in flavoxate hydrochloride. |
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